GAP43 anticorps (C-Term)

N° du produit ABIN3043685

L’anticorps Lapin Polyclonal anti-GAP43 a été validé pour WB et IHC. Il convient pour détecter GAP43 dans des échantillons de Humain, Rat et Souris. Il y a 10+ publications disponibles.

Aperçu rapide pour GAP43 anticorps (C-Term) (ABIN3043685)

Antigène: GAP43 (Growth Associated Protein 43 (GAP43))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GAP43 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-GAP43 anticorps

Épitope: AA 216-238, C-Term

Fonction: Anti-Neuromodulin GAP43 Antibody Picoband®

Séquence: KPKESARQDE GKEEEPEADQ EHA

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Neuromodulin GAP43 Antibody (ABIN3043685). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human GAP43, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Kosik, K. S., Orecchio, L. D., Bruns, G. A. P., Benowitz, L. I., MacDonald, G. P., Cox, D. R., Neve, R. L. : Human GAP-43: its deduced amino acid sequence and chromosomal localization in mouse and human. Neuron 1: 127-132, 1988. 2. Strittmatter, S. M., Fankhauser, C., Huang, P. L., Mashimo, H., Fishman, M. C. : Neuronal pathfinding is abnormal in mice lacking the neuronal growth cone protein GAP-43. Cell 80: 445-452, 1995. 3. Chen, B., Wang, J.-F., Sun, X., Young, L. T. : Regulation of GAP-43 expression by chronic desipramine treatment in rat cultured hippocampal cells. Biol. Psychiat. 53: 530-537, 2003.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-GAP43 (ABIN3043685)

Liu, Lv, Zhu, Wu, Zhang, Xu, Zheng, Zhao: "Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo." dans: Scientific reports, Vol. 7, pp. 39869, (2018) (PubMed).

Tan, Yu, Song, Zou, Xu, Liu: "Expression of MicroRNA-29a Regulated by Yes-Associated Protein Modulates the Neurite Outgrowth in N2a Cells." dans: BioMed research international, Vol. 2017, pp. 5251236, (2018) (PubMed).

Liu, Liu, Zeng et al.: "Chronic Unpredictable Mild Stress Causing Cardiac and Thoracic Spinal Cord Electrophysiological Abnormalities May Be Associated with Increased Cardiac Expression of Serotonin and Growth-Associated ..." dans: BioMed research international, Vol. 2018, pp. 8697913, (2018) (PubMed).

Yuan, Sun, Yu, An: "Human microvascular endothelial cell promotes the development of dorsal root ganglion neurons via BDNF pathway in a co-culture system." dans: Bioscience, biotechnology, and biochemistry, Vol. 81, Issue 7, pp. 1335-1342, (2017) (PubMed).

Zhu, Liu, Wang, Nie, He, Zhang, Liu, Su: "Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats." dans: Molecular medicine reports, Vol. 12, Issue 4, pp. 5691-700, (2016) (PubMed).

Zou, Ding, Shi, Xu, Gong, Zhang, Liu: "MicroRNA-29c/PTEN pathway is involved in mice brain development and modulates neurite outgrowth in PC12 cells." dans: Cellular and molecular neurobiology, Vol. 35, Issue 3, pp. 313-22, (2015) (PubMed).

Zou, Ding, Wang, Xiong, Peng, Du, Zhang, Liu, Gong: "MicroRNA-29A/PTEN pathway modulates neurite outgrowth in PC12 cells." dans: Neuroscience, Vol. 291, pp. 289-300, (2015) (PubMed).

Wang, Liu, Jin, Xu, Wang, Han, Yin: "Fructus Corni extract-induced neuritogenesis in PC12 cells is associated with the suppression of stromal interaction molecule 1 expression and inhibition of Ca2+ influx." dans: Experimental and therapeutic medicine, Vol. 9, Issue 5, pp. 1773-1779, (2015) (PubMed).

Chen, Wei, Nie, Lin, Tian, Liu, Yu, Cheng, Yan, Wang, Liu, Deng, Lai, Zhou, Zhang, Lin, Chen: "?-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice." dans: Brain research, Vol. 1552, pp. 41-54, (2014) (PubMed).

Nie, Deng, Yang, Liu, Zhang, Wen: "Axonal regeneration and remyelination evaluation of chitosan/gelatin-based nerve guide combined with transforming growth factor-?1 and Schwann cells." dans: Cell biochemistry and biophysics, Vol. 68, Issue 1, pp. 163-72, (2014) (PubMed).

Détails sur GAP43

Antigène: GAP43 (Growth Associated Protein 43 (GAP43))

Autre désignation: GAP43

Sujet:

Synonyms: Neuromodulin,Axonal membrane protein GAP-43,Growth-associated protein 43,Neural phosphoprotein B-50,pp46,GAP43,

Tissue Specificity: Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. .

Background: Growth Associated Protein-43 (GAP-43), also known as nerve growth-related growth peptide GAP43, shares a high degree of homology between the sequence of the human gene and the rat gene. GAP-43 is considered a crucial component of an effective regenerative response in the nervous system. Somatic cell hybrids demonstrate localization of the GAP-43 gene to human chromosome 3 and to mouse chromosome 16. GAP-43 has been termed a "growth" or "plasticity" protein because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration. GAP-43 regulates growth of axons and modulates the formation of new connections.

Sequence Similarities: Belongs to the neuromodulin family.

Poids moléculaire: 43 kDa

UniProt: P17677